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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: Biochem J. 2012 Jul 1;445(1):93–100. doi: 10.1042/BJ20120304

Figure 5. ePAB phosphorylation is not required to promote translation.

Figure 5

(A) Immature oocytes expressing MS2, MS2–ePAB or MS2–4×Ala-ePAB were co-injected with a non-adenylated PABP-dependent luc reporter mRNA containing MS2-binding sites and PABP-independent control mRNA (depicted in Supplementary Figure S1B at http://www.BiochemJ.org/bj/445/bj4450093add.htm). Half of the oocytes were matured, and control-adjusted fold-stimulation is depicted. (B) Lysates from (A) were immunoblotted for expression of MS2–ePAB fusions, which do not resolve as a doublet owing to the increase in mass (14 kDa) conferred by the MS2 protein fusion.