Skip to main content
. Author manuscript; available in PMC: 2014 Sep 23.
Published in final edited form as: Chembiochem. 2013 Sep 3;14(14):1845–1851. doi: 10.1002/cbic.201300270

Figure 5.

Figure 5

Chronoamperometric measurements of the inactivation (Figure 5A and B) and re-activation (Figure 5C and D) rate of cyanide-inhibited CODH ICh and CODH IICh. The inactivation rate of CODH IICh by cyanide was measured at −460mV (CO oxidation, Figure 5A) and −560mV (CO2 reduction, Figure 5B). A final concentration of 0.5 mM cyanide in the electrochemical cell was used to measure the half-life time for inactivation. Cyanide release from CODH IICh (Figure 5D) at −760mV is much faster than the instrumental response. Conditions: 25 °C, 0.2 M MES buffer (pH=7.0), and rotation rate, 3500 rpm.