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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: Sci Transl Med. 2013 Jun 19;5(190):190ra79. doi: 10.1126/scitranslmed.3005471

Fig. 5. ER expression in EBOV permissive cell lines.

Fig. 5

(A) Western blot analysis of Vero E6 and HepG2 cells treated with clomiphene, dimethyl sulfoxide (DMSO) (vehicle control), or no treatment (NT) and probed for either ER-α or ER-β expression. MCF-7 and SK-BR-3 cell lysates were used as controls for ER-α and ER-β, respectively. (B) eGFP-EBOV was used to infect cell lines both positive and negative for ER-α and/or ER-β expression (as shown in fig. S4 and summarized in Table 2) that were treated with clomiphene. (C) Clomiphene and toremifene treatment of eGFP-EBOV–infected HUVECs that do not express ER-α or ER-β (fig. S5). Results indicate that both compounds inhibit virus infection similarly to cells that express ER receptors.