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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: Nature. 2013 Jun 12;499(7456):50–54. doi: 10.1038/nature12318

Figure 4. Determinants of H2AK15ub recognition.

Figure 4

a, Upper panel: Sequence of the H2A N-termini of various mutants. Mutations are highlighted in color. Lower panel: GST-Tudor-UDR pulldown assays of RNF168-ubiquitylated NCPs (+) assembled with H4KC20me2 and the indicated H2A mutants. A reaction without E1 (−) was used as control. b, GST-Tudor-UDR pulldown assays of NPCs with the indicated H2A ubiquitylations. c, Pulldown assays of RNF168-ubiquitylated H4KC20me2-NCPs with the indicated fusion proteins. A white arrowhead highlights faint uH2A bands. d, GST-UDR pulldown assays of unmethylated NCPs that were either ubiquitylated with the indicated E3 or not (no E1).