Figure 3.

Respiration, inner membrane potential, and ROS production by liver and heart mitochondria of protocol I mice treated with control or HF diets for 12 weeks. Oxygen consumption and membrane potential as a function of the amount of ATP added are shown for liver mitochondria (panels A and B) and for heart mitochondria (panels C and D), respectively. Oxygen consumption and H₂O₂ production at selected 0 and 10 μM ATP are shown for liver and heart mitochondria in panels E and F, respectively. No significant differences in respiration, potential, or ROS production by diet intervention were observed for either liver or heart mitochondria. “mg” in y-axis (panels A, C, E, F) refers to mg mitochondrial protein.