Figure 8.

Retinal vasculature and vascular perfusion in the EpoR knockdown mice. A: Retinal flatmounts of EpoR^flox/flox and EpoR^flox/flox;Opn-Cre littermates at P84 and of the EpoR^flox/flox;α-Cre mice at P30. Vessels were stained with isolectin IB₄ coupled to Alexa 594. Shown are representative flatmounts of three animals per genotype. Scale bar: 500 µm. B: Staining of blood vessels on retinal cryosections (isolectin IB₄, coupled to Alexa 594) in the EpoR^flox/flox, EpoR^flox/flox;Opn-Cre, and EpoR^flox/flox;α-Cre mouse retinas at P84. Vessels were highlighted with artificial coloring (primary plexus (P): red; intermediate plexus (I): green; outer plexus (O): blue). Cell nuclei were stained with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) (white). Scale bar: 100 µm. C: Fluorescein angiography of the EpoR^flox/flox, EpoR^flox/flox;Opn-Cre, and EpoR^flox/flox;α-Cre mouse retinas at P84 performed with Micron III imaging. Shown are representative images of three animals per genotype.