Table 2.
Viability and apoptosis of cumulus cells per individual oocyte relative to several clinical parameters
| Clinical parameter | Live cells (%): Annexin V−/Propidium iodide-(M±SEM) | Annexin V+cells (%) (M±SEM) | n | Caspase-3/7 activity (Mean RLU±SEM) | n |
|---|---|---|---|---|---|
| Oocyte maturity and quality | |||||
| MII oocytes | 48.2±4.9 ** | 11.6±2.6 # | 16 | 2865±614 | 37 |
| MI + GV oocytes | 27.2±12.5 | 11.2±5.8 | 4 | 5090±1808 | 12 |
| AT + DEG oocytes | 17.3±6 ** | 25.8±6.5 # | 6 | 4807±2028 | 14 |
| Oocyte fertilization status | |||||
| Fertilized oocytes | 47.6±5.3 | 13.5±3.2 | 12 | 2885±437 * | 20 |
| Not fertilized oocytes | 50.2±13.1 | 6±2.2 | 4 | 1633±367 * | 16 |
| Patient age (year) | |||||
| ≤29 | 39.3±5.4 | 9.6±2.1*** | 19 | 6775±1831 ** | 17 |
| ≥30 | 33.7±9 | 29.1±4.3*** | 7 | 2591±547 ** | 46 |
#
p = 0.05
*
p < 0.05
**
p ≤ 0.01
***
p < 0.001
MII: Metaphase II; MI: Metaphase I; GV: Germinal Vesicle; AT: Atretic; DEG: Degenerated