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Oligonucleotide primers used in the PCR assay
| Primer | Nucleotide sequence (5’-3’) | PCR target | Expected size (bp) |
|---|---|---|---|
| IntI1F | ACGAGCGCAAGGTTTCGGT | ||
| IntI1R | GAAAGGTCTGGTCATACATG | Class 1 integrase gene | 565 |
| IntI2F | GTGCAACGCATTTTGCAGG | ||
| IntI2R | CAACGGAGTCATGCAGATG | Class 2 integrase gene | 403 |
| IntI3F | CATTTGTGTTGTGGACGGC | ||
| IntI3R | GACAGATACGTGTTTGGCAA | Class 3 integrase gene | 717 |
| 5'-CS | GGCATCCAAGCAGCAAG | ||
| 3'-CS | AAGCAGACTTGACCTGAT | Variable region of integrons | Uncertain |
