Table-I.

Setting of the tubes as per study protocol

Table	Rows
	I	II	III
	D/D Saline + Antisera	Controls Saline + Each dilution	Test Saliva + Each Dilution
1 (1:2)	+	+	+
2 (1:4)	+	+	+
3 (1:8)	+	+	+
4 (1:16)	+	+	+
5 (1:32)	+	+	+
6 (1:64)	+	+	+
7 (1:128)	+	+	+
8 (1:256)	+	+	+
9 (1:512)	+	+	+
10 (1:1024)	+	+	+

Antisera=100 µl, Saline=100 µl, Saliva=100 µl, Each dilution=100 µl, D/D = Double dilution