Figure 3.

Bufalin interfered with activation of NF-κB signaling in the carrageenan-induced paw edema model. In this experiment, the dose of bufalin was 0.6 mg/kg. (a) The level of IκBα was detected by western blot. The nuclear (b) and cytosolic (c) NF-κB p65 were monitored after 0.6 mg/kg bufalin injection by western blot. The levels of IκBα (d), nuclear (e), and cytosolic (f) p65 were analyzed using Image J.2x software. Data are expressed as the mean ± S.E.M. of 3 determinations, *P < 0.05, **P < 0.01, and ***P < 0.001, compared with the vehicle group. WCL: whole cell lysate, NL: nuclear lysate, and CL: cytosolic lysate.