Figure 3.

Water decoction of Taxus cuspidate induces a G1 cell cycle arrest on tumor growth. (a) Western blot analysis was used to measure the expression of cyclin D1 and CDK4 in cells derived from four representative implanted samples from each group. The band intensities were measured by densitometry and the relative indicated protein expression was shown. **P < 0.01 compared with the control group. (b) IHC staining with anti-cyclin D1 antibody was performed using sections of transplanted tumors from each group. Cyclin D1 was evaluated quantitatively by calculating the ratio of the cyclin D1-positive area to the total area in each field and the mean value from 5 fields under 200x microscopy was determined. Original magnification ×200. (c) Single-cell suspension prepared from transplanted tumor resection was analyzed by flow cytometry to determine the number of cells in G0/G1, S, and G2/M phases. (A) Control group, (B) low concentration group, (C) middle concentration group, and (D) high concentration group.