Table 5.
Oxidative stress and antioxidant responses in tissue of common barbel (Barbus barbus L.) yearlings fed on control diet containing Se-free microalgae biomass and on diets supplemented by either sodium selenite or Se-enriched microalgae (replacing Se-free microalgae) to obtain Se concentrations of 0.3 mg kg−1from sodium selenite (Se 0.3) or 0.3 and 1.0 mg kg−1from Se-enriched microalgae biomass Algadiet 0.3 and Algadiet 1.0, respectively.
| Indices | Tissues | Test groups | |||
|---|---|---|---|---|---|
| Control | Se 0.3 | Algadiet 0.3 | Algadiet 1.0 | ||
| TBARS (nmol g ww−1) | Muscle | 8.4 ± 3.8a | 7.5 ± 4.4a | 7.1 ± 3.6a | 6.5 ± 3.6a |
| Liver | 32.8 ± 9.1a | 33.3 ± 8.3a | 32.3 ± 9.8a | 31.4 ± 8.1a | |
| Intestine | 3.4 ± 1.1a | 3.1 ± 0.9a | 2.9 ± 0.8a | 2.8 ± 0.9a | |
|
| |||||
| SOD (U mg−1 protein) | Muscle | 21.9 ± 4.5a | 19.3 ± 5.4a | 20.5 ± 5.4a | 21.4 ± 4.2a |
| Liver | 141.9 ± 21.8a | 127.1 ± 21.2a | 137.0 ± 15.3a | 164.0 ± 26.3a | |
| Intestine | 5.1 ± 1.6a | 4.8 ± 1.1a | 5.0 ± 1.3a | 4.3 ± 1.2a | |
|
| |||||
| CAT (mU mg−1 protein) | Muscle | 2.4 ± 0.8a | 3.7 ± 0.8b | 2.7 ± 1.0a | 4.6 ± 1.1c |
| Liver | 26.9 ± 8.1a | 41.7 ± 8.6bc | 35.4 ± 7.7b | 47.1 ± 9.6c | |
| Intestine | 0.8 ± 0.3a | 0.9 ± 0.3a | 0.8 ± 0.2a | 0.8 ± 0.2a | |
|
| |||||
| GPx (U mg−1 protein) | Muscle | 66.4 ± 14.9a | 63.0 ± 10.7a | 64.8 ± 9.4a | 62.5 ± 8.5a |
| Liver | 194.4 ± 45.1a | 185.6 ± 35.4a | 177.4 ± 32.9a | 198.2 ± 26.0a | |
| Intestine | 29.6 ± 6.6a | 29.5 ± 7.6a | 27.9 ± 6.4a | 25.0 ± 6.3a | |
|
| |||||
| GR (U mg−1 protein) | Muscle | 17.4 ± 7.3b | 7.3 ± 3.2a | 10.0 ± 3.6a | 6.8 ± 2.8a |
| Liver | 6.0 ± 2.3a | 6.9 ± 2.0a | 6.2 ± 2.3a | 6.0 ± 2.6a | |
| Intestine | 8.1 ± 2.0a | 7.7 ± 2.7a | 7.1 ± 2.6a | 6.7 ± 2.5a | |
Different superscripts in the same row indicate significant differences α = 0.01; n = 8 per replication.