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Effects of brazilin on glucose-induced expression of p-eNOS and pERK in HUVECs: HUVECs (2 × 105 cells/well) were pretreated with a solvent control (0.1% DMSO) or brazilin (50 and 100 μM) for 2 h and then treated with glucose (50 mM) for 30 min to detect the phosphorylation of (a) p-eNOS and (b) p-ERK1/2. The β-actin was used as an internal control.
