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. 2014 Feb 27;2014:464217. doi: 10.1155/2014/464217

Figure 2.

Figure 2

Double staining of N-cadherin with smoothelin and PGP9.5. (a–d) Costaining of N-cadherin (green) and smoothelin (red) in the bladder wall shows no colocalization. (a) Transversal and (b) longitudinal sections. (c) N-cadherin+ structures intermingle with smooth muscle cells in the region of the muscularis mucosae (asterix) (d) Note the aggregation of multiple N-cadherin+ cells in the lamina propria (arrow). (e) PGP9.5 is expressed by a nerve trunk (red). (f) Aggregated N-cadherin+/PGP9.5− cells are closely associated with a ganglion region (asterisk). Ganglion coexpressesbackground signal of N-cadherin, resulting in orange bodies (arrowheads). Note high contrast to the punctate expression of N-cadherin in the N-cadherin+/PGP9.5− cells. DAPI (blue) for nuclei. Magnification ×400.