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Experimental design and classification of animal groups. I/R: ischemia/reperfusion animals; VEH: vehicle group (saline); ISO: isoflurane anesthesia (4% induction, 2% maintenance); CHLO: chloral hydrate anesthesia (300 mg/kg ip). MK801 group corresponded to animals who received MK801 IV injection (0.5 mg/kg) 3 minutes before MCAO.
