. Author manuscript; available in PMC: 2014 Mar 16.

Published in final edited form as: J Proteome Res. 2010 Feb 5;9(2):865–875. doi: 10.1021/pr900761m

Table 2.

Immunosuppressant-induced changes in kidney protein expression compared to vehicle controls as estimated based on 2D-gels.

Protein name	Acc. number	Score / AA-cov.	Changes compared to controls					MW on gel [kDa]	pI on gel [pH]	MW Theo- retical [kDa]	pI Theo- retical [pH]
Protein name	Acc. number	Score / AA-cov.	S	C	S/C	T	S/T	MW on gel [kDa]	pI on gel [pH]	MW Theo- retical [kDa]	pI Theo- retical [pH]
vimentin	14389299	233 / 32%	↑^*	↑↑^*	↑↑^*	=	=	56	4.9	54	5.1
caldesmon	6978589	175 / 25%	=	↑^*	↑^*	=	=	60	6.3	61	6.3
Prolyl-4-Hydroxylase	38197382	250 / 30%	=	↑^*	↑^*	=	=	50	4.9	60	4.8
Drebrin like protein	13786198	137 / 25%	↑^*	↑↑^*	↑^*	=	↑	50	4.9	48	4.9
Actinrelated Protein 3B	12835802	178 / 30%	↑	↑^*	↑↑^*	=	↑	48	5.7	47	5.6
HSP 90 beta	34862435	282 / 23%	=	↑^*	↑	=	=	47	4.7	92	4.7
Glycine amidinotransferase	13385454	91 / 20%	↓^*	=	=	=	↓^*	47	6.8	48	7.2
Angiogenin inhibitor	20806151	196 / 32%	=	↑^*	↑	=	=	45	4.6	49	4.6
Kidney aminoacylase	50925537	273 / 50%	↑^*	↑↑^*	↑↑^*	=	↑	45	6.3	45	6.1
Catalase	6978607	176 / 23%	↑^*	=	↑	↑	↑↑^*	40	6.1	59	7.5
Fructose-1,6-bisphosphatase	51036635	264 / 53%	=	↑^*	↑^*	=	=	39	5.6	40	5.5
NADH oxidoreductase	32996721	181 / 33%	↓↓^*	↓↓^*	↓↓^*	=	=	38	6.0	40	7.1
Pyridoxal-(Vit.B6)-kinase	13929082	209 / 51%	↑^*	↑^*	↑↑^*	↑	=	35	6.3	35	6.3
Pyruvate kinase	16757994	182 / 25%	↑^*	↑	↑↑^*	↑^*	↑	34	6.4	58	6.7
Regucalcin	13928740	160 / 41%	↓^*	↓↓^*	↓↓^*	=	=	33	5.3	33	5.3
Isocitrate DH (NADP)	13928690	196 / 30%	↓↓^*	↓^*	↓^*	=	=	33	6.7	47	6.6
alpha-2u-globulin, major urinary protein (7 spots)	22219450	100–180 / 21%– 51%	↓^*	↓^*	↓↓^*	=	=	22 - 20	5 - 7	20	5.8
GM2 activator Protein	25006237	95 / 18%	=	↑	↑^*	=	↑	~20	5.0	21	5.6
Proteasome subunit 3	8394082	87 / 34%	↑^*	↑	↑↑^*	=	↑	~20	6.4	23	6.2
Calbindin-D	14010887	208 / 46%	=	↓↓^*	↓↓^*	↓	↓	25	4.6	28	4.7
TCTP p21	55562869	55 / 23%	↑	↑	↑^*	↑	=	~20	4.8	20	4.8
Plastin 3 T-isoform	34875362	378 / 39%	=	↑	↑↑^*	↑	=	~60	5.2	70	5.2
Aldehyde DH 7 antiquintin	34879551	228 / 28%	=	↑	↑	↓	↓^*	55	6.8	55	7
Aldose reductase	6978491	163 / 42%	=	=	=	↓	↓↓^*	35	6.5	35	6.3
Ddah1 protein	38371755	177 / 45%	↑	=	↑	↑↑^*	↑↑^*	31	6.1	31	5.8
RICKEN cDNA 1810013B01	27721409	60 / 21%	=	=	=	=	↑^*	24	5.4	22	5.6

Arrows represent more than −30% (↓), −50% (↓↓), +40% (↑), +100% (↑↑) of change between the group averages in the density of protein spots as compared to untreated controls (significant changes p<0.05 [Tukey test pairwise comparison to controls]). Molecular weights (49), isoelectric points (pI) are shown both as measured on gels and as listed in the Swiss Prot and NCBInr databases.

Abbreviations: AA-cov.: amino acid coverage, Acc. Numbers: NCBInr accession number, C: cyclosporine, C/S: cyclosporine + sirolimus, S: sirolimus, T: tacrolimus, T/S: tacrolimus + sirolimus.