Table 3.
Association between immunosuppressant-induced changes in the expression of proteins involved in energy metabolism (A), expression of kidney aminoacylase (B) and the urine concentrations of their corresponding substrates and/or products.
| Control | Srl1 | CsA10 | CsA10 + Srl1 |
Tac1 | Tac1 + Srl1 |
||
|---|---|---|---|---|---|---|---|
| A | Pre-TCA cycle [‰ of total integral] |
13 | 144*↑ | 169*↑ | 189*↑ | 121*↑ | 30↑ |
| TCA cycle [‰ of total integral] |
173 | 100*↓ | 121↓ | 64*↓ | 129↓ | 114↓ | |
| Pyruvate kinase [% of control] |
100 | 180*↑ | 160↑ | 220*↑ | 140*↑ | 160↑ | |
| NADH oxidoreductase [% of control] |
100 | 30*↓ | 40*↓ | 30*↓ | 110 | 110 | |
| B | Hippurate [‰ of total integral] |
47.2 | 15.0*↓ | 13.1*↓ | 4.7*↓ | 23.3↓ | 21.9↓ |
| Kidney aminoacylase-1 [% of control] |
100 | 165*↑ | 190*↑ | 195*↑ | 100 | 155↑ | |
| C | α-glucose [‰ of total integral] |
4.0 | 2.3 | 28.9*↑ | 117.0*↑ | 4.8 | 2.9 |
| F-1,6-BP [% of control] |
100 | 120 | 187*↑ | 171*↑ | 103 | 106 | |
| D | Creatine [‰ of total integral] |
6.0 | 28.6*↑ | 2.5 | 28.9*↑ | 3.7 | 22.4*↑ |
| AGAT [% of control] |
100 | 61*↓ | 122 | 85 | 103 | 74*↓ |
Arrows mark the direction of concentration changes.
significant changes p<0.05 (Tukey test pairwise comparison to controls).
Abbreviations: CsA10: 10 mg/kg/d cyclosporine, CsA10+Srl1: 10 mg/kg/d cyclosporine+ 1 mg/kg/d sirolimus, Srl1: 1 mg/kg/d sirolimus, Tac1: 1 mg/kg/d tacrolimus, Tac1+Srl1: 1 mg/kg/d tacrolimus + 1 mg/kg/d sirolimus; Pre-TCA cycle metabolites: sum of lactate and acetate concentrations; TCA cycle metabolites: sum of succinate, citrate and 2-oxoglutarate concentrations; F-1,6-BP: Fructose-1,6-bisphosphatase, AGAT: Arginine:glycine-amidinotransferase.