Figure 4. PDK1 inhibitor GSK2334470 delays melanoma formation and inhibits metastases.
(a and b) representative H&E stain of skin (a) and lungs (b) in vehicle and GSK2334470 treated mice (BrafV600E::Pten−/−::PDK1−/−) following perinatal administration of 4-HT. Quantification shown for number of lung metastases is based on six sections 100 µm apart that were analyzed from three mice. Bar = 100 µm. (c) Representative S100 stain of lymph node in vehicle and GSK2334470 treated mice (BrafV600E::Pten−/−::PDK1−/−) following perinatal administration of 4-HT. Bar = 50 µm (d) Representative immunostaining of pAKT308 in the skin of vehicle and GSK2334470 treated mice following perinatal administration of 4-HT (BrafV600E::Pten−/−::PDK1−/−). Bar = 50 µm. (e) Representative S100 stain of lymph node in vehicle and GSK2334470 treated mice (BrafV600E::Pten−/−::PDK1−/−) following local administration of 4-HT. Bar = 50 µm. Quantifications shown on the right panels of panels c and e represent analyses from three animals for each group. Error bars represent SEM.