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(a) Analysis of antigen-binding affinity of scFv by ELISA to evaluate the activity of scFv towards IgA. (b) Competitive ELISA using the scFv and the anti-bovine IgA Mab. X-axis values are the concentrations of scFv and Y axis shows the optical density values. (c) Reactivity of scFv toward different immunoglobulins of bovine (IgA, IgG1, IgG2, and IgM) by indirect ELISA. (d) Reactivity of scFv toward saliva samples of cattle, buffalo, sheep, goat, and canine by indirect ELISA
