Figure 3.

(a) MTT assay for comparison of cytotoxic activities of AgNO₃ and [Ag(GSH)]⁺ towards HeLa cells and normal ones (L02 cells) treated with [Ag(GSH)]⁺ and used as control. HeLa cells were treated with AgNO₃ or [Ag(GSH)]⁺ at various concentrations (10, 40, 70, 100, 130, 160, 190, 220, 250 μg/mL) and L02 cells were treated with [Ag(GSH)]⁺ at various concentrations (10, 40, 70, 100, 130, 160, 190, 220, 250 μg/mL) for 24 h, and cytotoxicity was determined by the MTT method. (b) MTT assay assessment of time-dependent cytotoxicity towards HeLa cells and controls (L02 cells). HeLa cells were treated with AgNO₃ (40 μg/mL) or [Ag(GSH)]⁺ (130 μg/mL) and L02 cells were treated with [Ag(GSH)]⁺ (130 μg/mL) for different incubation times (12, 24, 36, 48 and 72 hours). Fluorescence excitation wavelength is 590 nm. Data were expressed as the means ± SD (standard deviation) from at least three independent experiments.