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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Ann Surg Oncol. 2013 Jul 31;20(12):3862–3868. doi: 10.1245/s10434-013-3168-2

Figure 2. MK-2206 inhibits phosphorylation of Akt.

Figure 2

Protein lysates of medullary thyroid cancer cells (MTC-TT) isolated after 4 days of treatment with MK-2206 (0–10 µM) demonstrate that increasing doses of MK-2206 reduce phosphorylation at the serine 473 residue of Akt (pAktS473), with marginal reduction at threonine 308 (pAktT308). Levels of total Akt protein remain stable with treatment. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control.