Figure 3.

Alizarin Red and Oil Red O staining of ADSCs and DFAT cells from subcutaneous fat. (A) ADSCs were cultured under osteogenic conditions for 4 weeks; Alizarin Red staining for matrix mineralization and quantification was performed. (B) DFAT cells were cultured under osteogenic conditions for 4 weeks; Alizarin Red staining for matrix mineralization and quantification was performed as described. (C) ADSCs were stained with Oil Red O to detect intracellular lipid accumulation. The lipid retained dye was eluted in isopropanol and quantified by spectrophotometric analysis. The dye is visualized at 10× under light microscopy with brightfield. (D) DFAT cells were stained as above and the lipid retained dye quantified as detailed. The differentiation was performed on four different dishes of cells with similar results. DFAT cells were passage 5 and ADSCs were passage 7. **p<0.05 vs. ADSC group. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/wound