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. 2014 Mar 1;3(3):219–228. doi: 10.1089/wound.2013.0452

Figure 5.

Figure 5.

Measurement of wound closure by electric cell-substrate impedance sensing (ECIS™). (A) Normalized capacitance over a time course for HDFs exposed to 48 h of conditioned media (CM) from ADSCs and DFAT cells, or unconditioned mesenchymal stem cell basal medium (MSC-BM). A capacitance of 1 indicates 0 coverage/confluence at the time of wounding (0). Data are represented as mean±SD (n=4 wells of HDFs per CM group). (B) The time taken for HDFs to reach a normalized capacitance of 0.6. (C) Normalized capacitance values of the HDFs at 5.5 h after wounding. (D) The HDF migration rate as calculated by v=r/t, where r=radius of the electrodes (125 μm) and t=time (hours to reach a normalized capacitance of 0.6). DFAT cells were passage 5 and ADSC were passage 7.