Figure 5.

The images show the migrating tongue of keratinocytes in an incisional human wound at 30 h after wounding. The cells were moving toward the right [large arrows in (A) and (B) indicate the direction of movement]. (A) The section was prepared for immunofluorescence using antibodies against α₃ integrin (red) and BPAG1e (green). The nuclei are stained with DAPI (blue). α₃ integrin (red) is predominantly at sites of cell-to-cell contact with no clear co-distribution with BPAG1e (green) located along the advancing wound margin juxtaposed to the wound matrix (arrows). (B) The section was costained with antibodies against β₄ integrin (red) and BPAG1e (green). The nuclei are stained with DAPI (blue). Note that β₄ integrin and BPAG1e co-distribute as evidenced by the yellow color along the wound margin (arrows). M, matrix of wound bed. These images were presented in Underwood et al. as Figs. 8C and 5C.¹⁰⁴ Scale bar, 10 μm.