Table 2.
Peptide mass mapping of the potential N-glycosylation sites in GGCX by MALDI-TOF and Q-TOF mass spectrometry
| Measured m/z | Measured peptide mass | Expected peptide mass | Corresponding residues | Glycosylation site | Digestions method |
|---|---|---|---|---|---|
| 1821.9355a | 1820.9355 | 1819.9911 | 452-466 | N459 | Chymotrypsin |
| 2373.1978 | 2372.1978 | 2371.1562 | 458-476 | N459 | Trypsin |
| 2433.2903 | 2432.2903 | 2431.2561 | 625-647 | N627 | Trypsin, Trypsin/CNBr |
| 2617.3433 | 2616.3433 | 2615.3449 | 601-622 | N605 | Trypsin/CNBr |
| 2646.3074 | 2645.3074 | 2644.4038 | 618-641 | N627 | Chymotrypsin |
| 2690.4270 | 2689.4270 | 2688.3936 | 623-647 | N627 | Trypsin, Trypsin/CNBr |
| 694.84 (2)b | 1387.68 | 1386.68 | 547-559 | N550 | Endoproteinase Glu-C |
| 811.71 (3) | 2432.13 | 2431.26 | 625-647 | N627 | Trypsin, Trypsin/CNBr |
| 897.48 (3) | 2689.44 | 2688.39 | 623-647 | N627 | Trypsin, Trypsin/CNBr |
| 939.44 (1) | 938.44 | 937.44 | 602-609 | N605 | Chymotrypsin |
| 1217.06 (2) | 2432.12 | 2431.26 | 625-647 | N627 | Trypsin, Trypsin/CNBr |
a
Top panel: deglycosylated peptide detected by MALDI-TOF MS according to the 1-Da shift theory.
b
Bottom panel: deglycosylated peptide detected by Q-TOF MS according to the 1-Da shift theory. Numbers in parentheses indicate the charges of the corresponding peptides.