Figure 3. Rtp801 – dependent NF – κB activation by cigarette smoke.

(a) Total Bal cells in Rtp801 wildtype mice intratracheally transduced with AAV5 – expressing IκB super repressor (SR) or β – gal (Lz) for 7 – 10 days in ambient air (RA) or exposed to CSk for 1 day (n = 5 – 6 mice in each group). (b) Effect of transfection with RTP801 – (RTP) or empty vector pcDNA3.1+ (pc) on NF – kB – dependent reporter Firefly luciferase activity (lower) in LPS, 1 µg ml⁻¹ or vehicle – (veh) treated rat lung endothelial cells (normalized by Renilla luciferase activity, AU; n = 3 independent experiments), and RTP801 mRNA expression in transfected cells (upper). (c) NF – κB reporter activity in wildtype (WT) or Rtp801 ^{− / −} (KO) MLF treated with LPS – (1 ug ml⁻¹) or 1% CSE (normalized AU; n = 3 independent experiments). (d) Expression levels of phosphorylated Ser536 p65 NF – κB (p – p65 NF – κB) in whole lung lysates of Rtp801 wildtype and Rtp801 ^{− / −} mice, exposed to CSk for 1 day (normalized by total p65, n = 3 – 5 mice in each group). (e) Vehicle (Ctl) and H2O2 ₋ induced (125 µM, 6 h) ROS levels in Rtp801 wildtype and Rtp801 ^{− / −} MEF. *: P < 0.05