Fig. 3.

Molecular signatures in sh-Jmjd3–transfected retina. (A) Plasmids encoding sh-Jmjd3 or sh-control with CAG-EGFP were introduced into the retina at E15; the retina was then cultured for 12 d as retinal explants, and RT-qPCR was performed. All values of qPCR were normalized by the normalization factor value calculated by housekeeping genes, Gapdh and Sdha. (B) Transition of expression of Bhlhb4 and Vsx1 was examined by RT-qPCR during retinal development. H3K27me3 (C) or H3K4me3 (D) modification of gene loci in purified Isl1-positive or Isl1-negative cells was examined by ChIP analysis. Experiments were done three times independently, and the average and SD are shown. Abcam antibody (ab6002) for H3K27me3 was used in C. **P < 0.01, *P < 0.05, and P > 0.05 (n.s.) were calculated by the Student t test.