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. 2014 Feb 24;111(10):3793–3798. doi: 10.1073/pnas.1324266111

Fig. 5.

Fig. 5.

Expression of proinflammatory cytokine IL-8 was mediated via TLR10 during influenza A virus replication. (A) Dual-luciferase assay: 293T cells were transfected with pPOLI-NS-Luc reporter and pRL-CMV control plasmid, together with the plasmids including the expression plasmids of PB2, PB1, PA and NP (RNP) and human TLR10 expression plasmid (TLR10). Cells were harvested at 20 h after transfection. Luciferase activity shown was the Firefly luciferase activity normalized to Renilla luciferase activity. (B) RT-PCR analysis of proinflammatory cytokines IL-8 mRNA expression. Similar to A, 293T cells were harvested at 20 h after transfection. Data shown are representative of two independent experiments and error bars indicate SD of technical triplicates.