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. 2014 Feb 24;111(10):3841–3846. doi: 10.1073/pnas.1314184111

Fig. 3.

Fig. 3.

Effect of AS exposure on fear response and kiss1 and serotonergic genes. (A) Schematic of treatment timeline. Fish were exposed with AS for 8 min for 7 d. Behavior was observed on the 1 d of AS exposure. Brain samples were collected from the fish treated with 1 d (Fig. S4) and 7 d AS exposure. (B) Side-view video-tracking of swimming behavior of a fish treated with AS for an 8-min recording. A test tank was divided into two equal virtual horizontal portions, marked by a white dividing line. (C and D) Graphs of behavioral parameters of fear response, including the number of erratic movements (C) and the freezing time (D) (n =20 per group). (E–H) Graphs showing the effect of 7 d exposure of AS (8 min/d) on mRNA levels of kiss1 (E), pet1 (F), slc6a4a (G), and tph2 (H) (n =20 per group). Data are presented as mean ± SEM. *P < 0.001, independent t-test comparisons between control and AS-exposed fish.