Skip to main content
. 2014 Feb 24;111(10):3739–3744. doi: 10.1073/pnas.1401861111

Fig. 3.

Fig. 3.

lst-1 and sygl-1 are targets of GLP-1/Notch activation. (A and B) lst-1 and sygl-1 mRNA expression in wild-type young adult gonads. (C and D) Expression of lst-1 and sygl-1 in GSCs within the niche requires GLP-1/Notch. Shown are L4 gonads. GLP-1(+): lst-1, 97% positive (n = 37); sygl-1, 97% positive (n = 36). GLP-1(-): lst-1, 0% positive (n = 28); sygl-1, 0% positive (n = 33). (E) Wild-type sygl-1 promoter drives reporter expression in germ cells within the niche (100% GFP-positive; n = 45), whereas 4× LBS mut sygl-1 promoter does not (0% distal positive; n = 46). Filled white triangles mark nuclei positive for H2B::GFP; empty white triangles mark nuclei negative for H2B::GFP. Because reporters were occasionally silenced, only germ lines expressing GFP proximally were scored (SI Materials and Methods). (A–E) Asterisk marks distal end.