Snail expression in PK-45H and KLM-1 cells. (A and C) Sh clones exhibited a significantly decreased (A) Snail mRNA and (C) protein expression compared with the Sc clones. (B and D) Nes clones exhibited a significantly increased (B) Snail mRNA and (D) protein expression compared with teh Mock clones. *P<0.05. (A and B) qRT-PCR. (C and D) Immunocytochemistry: green, Snail; blue, DAPI. Upper panels, image of Snail; lower panels, merged image. Original magnification, ×1000.