Figure 2.

Growth-inhibitory effect of single agent cisplatin (blue column) and docetaxel (red column), as well as the combination of BI2536/cisplatin (yellow column) and BI2536/docetaxel (black column) at a fixed cell line-specific concentration, as shown in Table I after 72 h treatment in PE/CA-PJ 49 cell line. The results of the other cell lines treated were similar to these. The corresponding untreated tumor cell line (grey column) served as the control and was incubated, according to the instructions of the suppliers, only with antibiotics at 37°C in the cell type-specific medium Quantum 263 with L-glutamine. The absolute tumor cell numbers in treated and control cell lines were determined in a Rosenthal chamber at 72 h after treatment or incubation with Quantum 263 (controls), respectively. Mean values of three independent experiments with standard deviation are shown. Single agent differences vs. combin ation treatment and untreated controls are indicated by asterisks. Combination treatment of BI2536 with cisplatin or docetaxel inhibited cell proliferation significantly higher than therapy with cisplatin or docetaxel alone (P≤0.015).