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. Author manuscript; available in PMC: 2014 Mar 17.
Published in final edited form as: Anal Chem. 2012 Mar 7;84(6):2776–2783. doi: 10.1021/ac203143y

Figure 3.

Figure 3

Affinity maturation of phage-displayed, PSMA-binding ligands. (a) Phage-based ELISA with the first generation PSMA-1 and PSMA-2 ligands. Throughout this report, error bars in ELISAs indicate standard error (n=3). (b) The homolog-shotgun scanning library design used the PSMA-2 sequence as a template with the programmed mutations shown in red. The selectants incorporated the mutations highlighted in red. (c) Phage-based ELISA with the affinity-matured, PSMA-binding selectants. (d) Phage-based ELISA comparing PSMA-1,-2, and -3.