Table 3.
Amino acid compositions and molecular masses of Bc-CPs. Observed values are obtained with ninhydrin-based analysis and MALDI-ToF mass spectrometry, whereas predicted values are derived from translated cDNA sequences. For the observed values, the maximum standard deviation is ~5% of the values shown.
| CP-1a | CP-1b | CP-2 | CP-3 | |||||
|---|---|---|---|---|---|---|---|---|
| Observed | Predicted | Observed | Predicted | Observed | Predicted | Observed | Predicted | |
| Ala | 9.0 | 8.5 | 9.0 | 8.9 | 6.4 | 5.7 | 6.3 | 5.6 |
| Arg | 4.7 | 4.5 | 4.7 | 4.5 | 4.2 | 3.1 | 4.1 | 3.3 |
| Asx | 10.1 | 11.5 | 10.1 | 11.6 | 10.8 | 12.9 | 10.7 | 11.8 |
| Cys | 0.0 | 0.7 | 0.0 | 0.7 | 0.0 | 0.4 | 0.0 | 0.4 |
| Glx | 18.6 | 16.2 | 18.6 | 16.9 | 16.1 | 14.8 | 16.7 | 14.8 |
| Gly | 5.1 | 4.9 | 5.1 | 4.9 | 5.9 | 5.9 | 6.3 | 5.9 |
| His | 2.2 | 1.9 | 2.2 | 2.1 | 3.4 | 3.7 | 3.7 | 3.7 |
| Ile | 5.9 | 7.5 | 5.9 | 6.8 | 5.4 | 6.6 | 6.1 | 6.5 |
| Leu | 9.5 | 9.6 | 9.5 | 9.9 | 9.1 | 9.4 | 8.6 | 9.3 |
| Lys | 12.0 | 10.6 | 12.0 | 10.4 | 12.2 | 11.4 | 12.6 | 11.5 |
| Met | 0.0 | 0.7 | 0.0 | 0.7 | 0.0 | 0.7 | 0.0 | 0.7 |
| Phe | 2.4 | 2.1 | 2.4 | 2.1 | 4.1 | 5.9 | 5.6 | 6.3 |
| Pro | 0.9 | 0.5 | 0.9 | 0.2 | 2.9 | 0.9 | 1.6 | 0.9 |
| Ser | 7.2 | 8.2 | 7.2 | 7.8 | 7.9 | 7.9 | 7.5 | 8.8 |
| Thr | 5.0 | 5.4 | 5.0 | 5.6 | 4.7 | 3.9 | 3.5 | 3.9 |
| Trp | - | 0.0 | - | 0.0 | - | 0.2 | - | 0.2 |
| Tyr | 0.3 | 0.0 | 0.3 | 0.0 | 0.5 | 0.0 | 0.0 | 0.0 |
| Val | 7.1 | 7.1 | 7.1 | 6.8 | 6.4 | 6.6 | 6.7 | 6.5 |
| Total | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| CP-1a | CP-1b | CP-2 | CP-3 | |||||
| Observed | Predicted | Observed | Predicted | Observed | Predicted | Observed | Predicted | |
| Mass (Da) |
47461 | 46880 | 47461 | 46962 | 51550 | 51437 | 52129 | 52016 |