RT-PCR of MSMB-NCOA4 fusion transcripts in human tissues and cancer cell lines. cDNA prepared from human prostate normal tissue (HPNT), human prostate tumor tissue (HPTT), human trachea normal tissue (HTNT) and 9 prostate cell lines was subjected to RT-PCR using two primer sets, the 5′MSMB exon1 primer (forward)/3′NCOA4 exon10 (3′UTR, reverse) (2,027 bp), and the 5′MSMB exon 1 primer/3′NCOA4 exons 5–6 (reverse) (643 bp)