Fig. 5.

Inhibition of C6-1 cell growth by knockdown of IL-33 receptor ST2. (A). C6-1 cells were treated with control lentivirus (sh-ctrl) or lentivirus particles encoding shRNAs against ST2 (lenti-sh-ST2_529 and lenti-sh-ST2_771), and then subjected to Q-PCR for ST2 mRNA expression. ST2 mRNA expression was significantly reduced in C6-1 cells after infection by lenti-sh-ST2_529 or lenti-sh-ST2_771 (ST2-KD1 or ST2-KD2). (B). The cell proliferation of ST2-KD1 or ST2-KD2 was examined using MTT assay at the indicated time points. ST2-KD1 and ST2-KD2 proliferated with a lower rate when compared with that of mock cells. (C). Mock, ST2-KD1 and ST2-KD2 were subjected to the colony formation assay. The number of ST2-KD formed colonies was significantly reduced. Data are means ± SEM of at least 3 independent experiments. **P < .01 versus mock. Scale bar in C, 5 mm.