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. 2014 Mar 17;8:9. doi: 10.3389/fnana.2014.00009

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Copyright © 2014 Salegio, Streeter, Dube, Hadaczek, Samaranch, Kells, San Sebastian, Zhai, Bringas, Xu, Forsayeth and Bankiewicz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Perivascular transport and pattern of transgene expression. (A–F) Immunostaining against GFP and blood vessels (CD31; endothelial marker) revealed the presence of GFP-positive cells surrounding blood vessels. Transgene expression was frequently observed to form rosette-like patterns in white matter, as demonstrated in cross-sectional views of blood vessels (B,C,E,F), indicative of perivascular transport of AAV particles irrespective of the serotype. Scale bars: A,B,D,E = 500 μm; C,F = 250 μm.