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. 2013 Dec 8;69(4):1017–1026. doi: 10.1093/jac/dkt467

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© The Author 2013. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Inhibition of in vitro DSE. (a) SDS–PAGE showing FimC:FimH DSE with FimG_Nte after a 12 h incubation. FimH:FimG_Nte forms an SDS-stable complex that shows a mobility shift compared with FimH. (b) Structure of AL1. (c) Concentration–response curve for in vitro FimC:FimH–FimG_Nte DSE in the presence of AL1 (0.001–2 mM). DSE progression as a function of AL1 concentration, presented as adjusted volume of pixels of the DSE product (FimH:FimG_Nte) over the adjusted volume of pixels of the total protein (FimC + FimH + FimH:FimG_Nte) and normalized over non-treated DSE.