Figure 1.

Screening for compounds that inhibit late stages of HIV-1 replication. 293T cells were transiently transfected with pNL4-3ΔEnvGFP and pX4 and then some wells were treated with epoxomicin (1.8 μM). Eighteen hours after treatment, RNA was extracted from culture supernatant, cDNA was synthesized and HIV-1 RNA-specific qPCR was performed. Supernatant from untreated cells was used as a control. No-template controls and no-reverse-transcription (RT) controls were also included. This figure appears in colour in the online version of JAC and in black and white in the print version of JAC.