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(a)
| Name of the oligonucleotides | Oligonucleotides sequence (5′-3′) | Annealing temperature (°C) |
|---|---|---|
| External primer T7 | CTAATACGACTCACTATAGGGC | 66 |
| Internal primer NotRsa | AGCGTGGTCGCGGCCGAGGT | 68 |
| GAPDH for. GAPDH rev. |
GTGGACGGCCCCTCTGC TCTGGTGGGCACCACGG |
63 |
| Suppression adapter (equimolar mixture of preliminary annealed oligonucleotides T7NotRsa and Rsa_10) | T7NotRsa CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGT Rsa_10 ACCTGCCCGG |
|
| Tag no. 89 | GTACTTTAATGGATGATCTCC | 56 |
| Tag no. 81 | GTACAAAAAGACTGCTGTTCC | 60 |
