Figure 5.

Effect of p40₂ and IL-12p70 on the expression of interleukin-7 (IL-7) in primary microglia isolated from wild-type, IL-12Rβ1^−/− and IL-12Rβ2^−/− mice. Microglia isolated from B6.129 wild-type, IL-12Rβ1^−/− and IL-12Rβ2^−/− mice were stimulated with 10 ng/ml of p40₂ and IL-12p70, under serum-free condition. After 4 hr of stimulation, the mRNA expression of IL-7 was monitored by semi-quantitative RT-PCR (a) and real-time PCR (b). After 18 hr of stimulation by 10 ng/ml IL-12p70, the expression of IL-7 protein was monitored by immunofluorescence (c–e). DAPI was used to visualize the nucleus. Results represent three independent experiments. *P < 0·001 versus control; NS, non significant.