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. 2014 Mar 11;141(4):617–627. doi: 10.1111/imm.12225

Figure 2.

Figure 2

Intratracheal (i.t.) exposure to O1-10 Fabs reduces the infiltration of neutrophils and production of interleukin-1β (IL-1β) in the lung tissue of mice with asthmatic responses. Mice sensitized (S) with ovalbumin (OVA) on days 0 and 14 were i.t. challenged (C) with 200 μg OVA on days 28, 29 and 30, and with 40 μg on day 35 as shown in Fig. 1(a). One hundred and twenty micrograms of O1-10 Fabs (S-C-O1-10 Fabs) and control Fabs (S-C-control Fabs) were i.t. administered once 30 min before the fourth challenge with OVA on day 35. Non-sensitized (NS) mice given OVA were also i.t. treated with O1-10 Fabs (NS-C-O1-10 Fabs). Twenty-four hours after the fourth antigenic challenge, histological changes in the lung (a–c, ×40) were examined by haematoxylin and eosin staining. Neutrophils and eosinophils are shown by red and black arrows, respectively. Gr-1+ cells (e–g, ×40) and IL-1β (i–k, ×20) in the lung were also immunohistochemically stained. Scale bars in all photographs represent 100 μm. Neutrophil infiltration and IL-1β production in the lung tissue were also scored as shown in Materials and methods (d, h, l). Data are means ± SEM of five mice. *P < 0·05 versus S-C-control Fabs. Data are representative of two independent experiments.