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. Author manuscript; available in PMC: 2015 Jan 17.
Published in final edited form as: ACS Chem Biol. 2013 Nov 19;9(1):227–236. doi: 10.1021/cb400581f

Figure 6.

Figure 6

Binding of the TPP riboswitch to 33P labeled OxyTPP. The Kd value was measured after 2 hr incubation of binding reactions containing TPP buffer (methods section), 100 nM 33P labeled OxyTPP and various concentrations of the TPP riboswitch (0.1, 0.5, 1, 5, 10, 30, 50 μM).