TABLE 1.

Primary Antibodies

Antigen	Immunogen	Manufacturer, purification, species, type, cat. no.	Concentration/dilution used
D2αPan receptor	Two synthetic peptides, aa 33–47 and aa 546–561 from P. interruptus as described in Clark et al. (2008)	21st Century Biochemicals, affinity-purified rabbit polyclonal, custom	2 μg/ml
D1αPan receptor	Synthetic peptide, aa 329–344 from P. interruptus as described in Clark et al. (2008)	Bethyl Laboratories, affinity-purified rabbit polyclonal, custom	1 μg/ml
D1βPan receptor	Synthetic peptide, aa 80–99 from P. interruptus as described in Clark et al. (2008)	Alpha Diagnostic, affinity-purified rabbit polyclonal, custom	1 μg/ml
Synapsin	Drosophila SYNORF1 aa 89–449GST fusion protein, representing the conserved 5′ end of synapsin cDNA, as defined in Klagges et al. (1996).	Developmental Studies Hybridoma Bank (University of Iowa), mouse monoclonal, cat. no. 3C11 concentrate	1:200
Synaptotagmin	Recombinant protein for aa 134–474 from Drosophila melanogaster as described in Littleton et al. (1993)	Gift from Dr. Hugo Bellen, rabbit polyclonal serum named DSTY2.	1:1,000
Tyrosine hydroxylase	Tyrosine hydroxylase purified from rat PC12 cells; recognized epitope is from conserved midportion of molecule	ImmunoStar, mouse monoclonal, cat. no. 22941	1:200