Figure 3.
(A–C) Effect of osteogenic differentiation by LMWP-TAZ fusion protein.
Notes: The hMSCs were treated with fusion proteins for indicated times in osteogenic media containing 20% fetal bovine serum, 50 µg/mL ascorbic acid, 10 mM β-glycerophosphate, and 10−7 M dexamethasone for detection of osteogenic differentiation. (A) The expression levels of specific osteogenic genes were detected by reverse-transcription polymerase chain reaction, and (B) quantified at 3 days (black column) and 7 days (white column). (C) Detection of matrix mineralization by alizarin red S. Three independent experiments were performed in duplicate, and significant differences are denoted by asterisks (P<0.05), indicating significantly different to time-matched TAZ group.
Abbreviations: mRNA, messenger ribonucleic acid; ALP, alkaline phosphatase; OCN, osteocalcin; OPN, osteopontin; PBS, phosphate-buffered saline; LMWP, low-molecular-weight protamine; Runx2, runt-related transcription factor 2; TAZ, PDZ-binding motif; hMSCs, human mesenchymal stem cells; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.