. 2014 Jan 8;5(2):311–320. doi: 10.4161/viru.27605

Table 1. Bacterial strains, plasmids, and primers used in study.

Strains	Genetic information	Background	References
SB300	Salmonella Typhimurium; Sm^r	Wild type	24
M1525	Salmonella Enteritidis P125109 wild type; Sm^r	Wild type	48 and 49
MT12	S. Typhimurium ΔssaV; Sm^r	SB300	This study
MT14	S. Typhimurium hha::aphT; Sm^r, Km^r	SB300	This study
MT15	S. Typhimurium hha::aphT, ΔssaV; Sm^r, Km^r	MT12	This study
Plasmids	Relevant genotype (S) and/or phenotype (S)	Resistance	References
pKD4	Template plasmid; FRT-aphT-FRT	Km^r	45
pKD46	Red recombinase expression plasmid; P_araB; oriR101	Amp^r	45
pCP20	FLP recombinase expression plasmid	Cm^r, Amp^r	45
pM973	bla PssaH gfpmut2 plasmid with oripMB1	Amp^r	22
Primers	Sequence (5′ to 3′)
Fw-hha	AGGCAGATAA CACCTGCGTG TTCTCTAAAA AGTAATGTAG CGTGATGTGT AGGCTGGAGC TGCTT
Rw-hha	GTTAGTTTGT CTTGTTAAAA ATTATTACAA TCATAGGTAG AATTTATATG AATATCCTCC TTAGT
Conf-hha	GTGATCTATA TCACTGTTCT ATAATAGCC
Fw-ssaV	TCATCGACAA ATAAAATTTC TGGAGTCGCA ATGCGTTCAT GGTTATGTGT AGGCTGGAGC TGCTT
Rw-ssaV	ATTTCAGCCT CAGACGTTGC ATCAATTCAT TCTTCATTGT CCGCCATATG AATATCCTCC TTAGT
Conf-ssaV	GCAATGAGTT GTTCTCCACC