Figure 1. The combination of rapamycin and resveratrol inhibits PI3K/Akt and mTOR signaling pathways. (A) WT MEFs and TSC2^−/− MEFs were treated with 20 nM rapamycin and/or 100 µM resveratrol for 24 h. Cells were lysed and the indicated proteins were detected by immunoblot. (B) ELT3 TSC2 o/e and ELT3 cells were treated as described in (A) and protein levels were detected by immunoblot. (C) 621–101 cells were treated as described in (A), and protein levels were detected by immunoblot. (D) 621–101 and NHBE cells were treated as described in (A), and protein levels were detected by immunoblot.