Figure 5. Analysis of H2A.Z recruitment on DNA damage in living cells. Nucleus of U2OS cells transfected with 53BP1-GFP (A) or H2A.Z-GFP (B) plasmids were irradiated with pulsed laser (532 nm) to induce DNA damage then images were collected sequentially. (C) Nucleus of U2OS cells first transfected with siCtrl or sip400 then transfected with 53BP1-GFP plasmid and irradiated to induce DNA damage as in (A). Time after irradiation is indicated above the photographs.