Figure 5. Uptake and release of FFAs Differentiated BMSC were serum-starved for 16 h, then cells were treated with 10 µM BODIPY FL C16 for 3 h (T0 starting point). Subsequently, cultures were incubated for 2 and 4 h (T1 and T2) with complete medium (α-MEM containing 10%FBS and bFGF) in presence of 3 µg/mL of ritrodrine chlorohydrate. The picture shows representative fields of BMSC containing fluorescent palmitate (BODIPY FL C16) at different time points. The graph shows the uptake levels of palmitate in different experimental conditions. Data are expressed as arbitrary units. The table shows the percentage of released palmitate that was calculated as the ratio between the BODIPY level at 2 or 4 h and the starting point (T1/T0 or T2/T0). Scale bar: 30 µM.