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. 2014 Mar 17;9(3):e90028. doi: 10.1371/journal.pone.0090028

Table 3. Reasons samples failed QA/QC.

DISEASE TERM % TUMOR NUCLEI (>75% PASS) % TUMOR NECROSIS (<20% PASS) TOTAL YIELD ≥20 NG (IN 14 UL) (PASS) NANODROP 260/280 ≥1.8 (PASS) BIOANALYZER RIN ≥8.0 (PASS) REASON FOR SAMPLE FAILURE
Bladder transitional cell carcinoma-trigonal (0204) 25–50% Fail <20% 3.91 ng/ul 3.76 N/A Sample too small for analysis
Lymphoma (0503) 75–100% >20% Fail 3092.04 ng/ul 2.03 9.40 High degree of sample necrosis
Osteosarcoma (appendicular) (0506) 0–24% Fail <10% 69.99 ng/ul 2.08 9.60 Too little viable tumor to analyze
Mast cell tumor (0507) 75–100% <10% 614.62 ng/ul 2.10 2.60 Fail Poor quality RNA due to abundant connective tissue
Melanoma-mucosa/mandible (0502) 0–24% Fail <10% 536.99 ng/ul 2.08 8.2 Sample too small for analysis
Lymphoma (1301) 0–24% Fail N/A 16.67 ng/ul 1.93 9.60 Sample too small for analysis
Histiocytic Sarcoma (1302) 0–24% N/A 657.76 ng/ul 2.12 9.80 Non-cancer diagnosis. Histopathology was panniculitis