Table 3. Reasons samples failed QA/QC.
DISEASE TERM | % TUMOR NUCLEI (>75% PASS) | % TUMOR NECROSIS (<20% PASS) | TOTAL YIELD ≥20 NG (IN 14 UL) (PASS) | NANODROP 260/280 ≥1.8 (PASS) | BIOANALYZER RIN ≥8.0 (PASS) | REASON FOR SAMPLE FAILURE |
Bladder transitional cell carcinoma-trigonal (0204) | 25–50% Fail | <20% | 3.91 ng/ul | 3.76 | N/A | Sample too small for analysis |
Lymphoma (0503) | 75–100% | >20% Fail | 3092.04 ng/ul | 2.03 | 9.40 | High degree of sample necrosis |
Osteosarcoma (appendicular) (0506) | 0–24% Fail | <10% | 69.99 ng/ul | 2.08 | 9.60 | Too little viable tumor to analyze |
Mast cell tumor (0507) | 75–100% | <10% | 614.62 ng/ul | 2.10 | 2.60 Fail | Poor quality RNA due to abundant connective tissue |
Melanoma-mucosa/mandible (0502) | 0–24% Fail | <10% | 536.99 ng/ul | 2.08 | 8.2 | Sample too small for analysis |
Lymphoma (1301) | 0–24% Fail | N/A | 16.67 ng/ul | 1.93 | 9.60 | Sample too small for analysis |
Histiocytic Sarcoma (1302) | 0–24% | N/A | 657.76 ng/ul | 2.12 | 9.80 | Non-cancer diagnosis. Histopathology was panniculitis |